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Principles and Processes In Biotechnology

Board » Bihar Board » Class 12th » English Medium » Science » Biology » L-11 : Principles and Processes In Biotechnology

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ID- 6883
BSEB, 2019 (C)

1. Recombinant Protein 'Hirudin' has therapeutic use as:

  • (A) Antivenom
  • (B) Anticoagulant
  • (C) Antibiotics
  • (D) All of these
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ID- 6884
BSEB, 2019 (C)

2. Which organ is known as reservoir to store erythrocytes?

  • (A) kidney
  • (B) Heart
  • (C) spleen
  • (D) All of these
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ID- 6886
BSEB, 2020 (A)

3. Recombinant DNA vaccine is:

  • (A) immunogenic lipid
  • (B) immunogenic acid
  • (C) immunogenic protein
  • (D) exogenic protein
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ID- 6889
BSEB, 2020 (A)

4. 'GAATTC' is recognition site for which of the following?

  • (A) Eco RI
  • (B) Eco RII
  • (C) Hind II
  • (D) Bam HI
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ID- 6909
BSEB, 2012

5. Restriction enzymes are:

  • (A) Exonuclease
  • (B) Endontuclease
  • (C) Ligase
  • (D) Polymerase
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ID- 6910
BSEB, 2015, 2019 (C), 2021 (A)

6. Source of Eco Ri enzyme is:

  • (A) Bam HI
  • (B) E. coil
  • (C) both 'A' and 'B'
  • (D) Hind III
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7. Who discovered Restricted endonculease enzymes?

  • (A) Smith and Nathne
  • (B) Baiger
  • (C) Waxman
  • (D) Fleming
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ID- 6912

8. DNA is obtained for DNA finger printing:

  • (A) WBC
  • (B) Hair roots cells
  • (C) Body fluids
  • (D) All of these
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ID- 6913

9. Golden rice is a promising transgenic crop when released for cultivation, it will help in:

  • (A) High lysin content
  • (B) Pest resistance
  • (C) High protein content
  • (D) High vitamin A content
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ID- 6914

10. Plasmid is a:

  • (A) Fungus
  • (B) Plasmid
  • (C) Part of plasma membrane
  • (D) Extra chromosomal DNA in bacterial cell
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ID- 6915
BSEB, 2018 (C)

11. The total gene and their alleles in a population is known as:

  • (A) Gene pool
  • (B) Gene Bank
  • (C) Gene flow
  • (D) Genetic drift
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ID- 6916
BSEB, 2018 (C)

12. The first human hormone produced by recombinant DNA technology is:

  • (A) Estrogen
  • (B) Thyroxine
  • (C) Progesterone
  • (D) Insulin
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ID- 6917
BSEB, 2018 (C)

13. Biolistics is suitable for:

  • (A) Transformation of plant cell
  • (B) DNA finger printing
  • (C) Disharming pathogen vector
  • (D) Construction recombinant DNA by joining with vector
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ID- 6918
BSEB, 2019 (A)

14. Restriction enzymes are known as:

  • (A) Biological guns
  • (B) Molecular scissors
  • (C) Plasmid
  • (D) Micro pipette
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ID- 6919

15. Plasmid used to construct the first recombinant DNA was isolated from which bacterium spices?

  • (A) Escherichia coli
  • (B) Salmonella typhimurium
  • (C) Agrobacterium tumefaciens
  • (D) Thermus acquaticus
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EXPLANATION:

The first recombinant DNA was constructed by Stanley Cohen and Herbert Boyer in 1972. They cut the piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium Salmonella typhimurium and linked it to the plasmid of Escherichia coli.

Therefore option A is correct.

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ID- 6920

16. The term 'molecular scissor' refers to:

  • (A) recombinant DNA
  • (B) restriction enzymes
  • (C) Taq polymerase
  • (D) Palindromic nucleotide sequences
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ID- 6921

17. Who is the father of genetic engineering?

  • (A) Steward Linn
  • (B) Stanley Cohen
  • (C) Paul Berg
  • (D) Kary mullis
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ID- 6922

18. The term 'chemical knife' refers to:

  • (A) polymerases
  • (B) endonucleases
  • (C) ribonucleases
  • (D) cellulases
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ID- 6923

19. DNA cannot pass through a cell membrane as:

  • (A) it is too big to cross the membrane
  • (B) it is a hydrophilic molecule
  • (C) membrane does not have specific proteins to facilitate the transport
  • (D) None of the above
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ID- 6924

20. The letter 'R' in Eco R I is derived from:

  • (A) the name of genus
  • (B) the name of strain
  • (C) the name of spices
  • (D) the term 'restriction'
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ID- 6925

21. How many fragments will be generated if you digest a linear DNA molecule with a restriction enzyme having for recognition sides on the DNA?

  • (A) 3
  • (B) 6
  • (C) 5
  • (D) 4
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ID- 6932

22. How many fragments will be generated on the digestion of a closed circular DNA molecule with a restrictions enzyme having six recognition site on the DNA?

  • (A) 5
  • (B) 7
  • (C) 6
  • (D) 9
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23. The source of the restriction enzyme Hind III is:

  • (A) Escherichia coli RY 13
  • (B) Haemophilus influenzae Rd
  • (C) Bacillus amyloliquefaciens H
  • (D) Streptomyces albus
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ID- 6934

24. Which of the following is required for micro-injection method of gene transfer?

  • (A) Micro-particles
  • (B) Micro-pipettes
  • (C) Divalent cations
  • (D) Uv radiation
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ID- 6935

25. Which of the following is not a cloning vector?

  • (A) Cosmid
  • (B) pBR322
  • (C) Sall
  • (D) Phagemid
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EXPLANATION:

Sall is a restriction enzyme isolated from Streptomyces albus.

Therefore, option C is correct.

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ID- 6936

26. Which of the following bacteria is used as a vector for plant genetic engineering?

  • (A) Agrobacterium tumefacinens
  • (B) Bacteriophages
  • (C) Thermus aquaticus
  • (D) Pyrococcus furiosus
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ID- 6937

27. The first restriction endonuclease isolated was:

  • (A) Eco RI
  • (B) Bam HI
  • (C) Sal I
  • (D) Hind II
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ID- 6938

28. Viral genome incorporated into host DNA is called:

  • (A) prophase
  • (B) prophage
  • (C) bacteriophage
  • (D) none of these
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ID- 6939

29. Which of the following is not a tool of genetic engineering?

  • (A) Cloning vector
  • (B) Restriction enzymes
  • (C) Foreign DNA
  • (D) GMO
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ID- 6957

30. In recombinant DNA technology, a plasmid vector is cleaved by:

  • (A) modified DNA ligase
  • (B) a heated alkaline solution
  • (C) the same enzyme that cleaves the donor DNA
  • (D) the different enezyme than that cleaves the donor DNA
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ID- 6958

31. Which of the following microbes transform normal plant and animal cells to cancerous cells respectively?

  • (A) Retroviruses and Rhizovium
  • (B) Escherichia coil and Agrobacterium tubefaciens
  • (C) Agrobacterium tubefaciens and Retroviruses
  • (D) Agrobacterium tubefaciens and A. rhizogens
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ID- 7101

32. Primers are:

  • (A) chemically synthesized oligonucleotides that complementary to the regions of DNA
  • (B) chemically synthesized oligonucleotides that are not complementary to the regions of DNA
  • (C) chemically synthesized, autonomously replicating circular DNA molecules
  • (D) specific sequences present on recombinant DNA
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ID- 7102

33. Enzyme 'Taq polymerase' used in PCR, has been isolated from bacterium:

  • (A) Agrobacterium tubefaciens
  • (B) Thermus aquaticus
  • (C) Streptomyces albus
  • (D) Escherichia coil
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ID- 7103

34. Which of the following is required to perform polymerase chain reaction?

  • (A) Primers, dNTPs and DNA polymerase
  • (B) DNA, CaCI2 and nuclease
  • (C) Mg+2, DNA
  • (D) Both 'A' and 'C'
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ID- 7104

35. In isolation of genetic material, chemical used to precipitate out the purified DNA is:

  • (A) bromophenol blue
  • (B) chilled ethanol
  • (C) ethidium bromide
  • (D) both 'A' and 'C'
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36. The term 'chimeric DNA' refers to:

  • (A) DNA with overhanging stretches
  • (B) DNA with palindromic sequence
  • (C) a recombinant DNA
  • (D) molecular scissors
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ID- 7106

37. A device in which large volume of living cells are cultured in order to get a specific product is called:

  • (A) PCR
  • (B) agitator
  • (C) bioreactor
  • (D) assimilatoe
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ID- 7107

38. Which of the following techniques can be used to detect genetic disorders in humans?

  • (A) PCR
  • (B) Gel electrophoresis
  • (C) Chromatography
  • (D) Spectroscopy
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ID- 7108

39. Rising of sought is due to:

  • (A) multiplication of yeast
  • (B) production of CO2
  • (C) emulsificiation
  • (D) hydrolysis of wheat flour starch into sugar
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ID- 7178

40. An enzyme catalysing the removal of nucleotides from the ends of DNA is:

  • (A) endonucleases
  • (B) exonuclease
  • (C) DNA ligase
  • (D) Hin - II
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ID- 7179

41. 'Restriction' in restriction enzyme refers to:

  • (A) cleaving of phosphodiester bond in DNA by the enzyme
  • (B) cutting of DNA at specific position only
  • (C) prevention of the multiplication of bacteriophage in bacteria
  • (D) all of the above
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ID- 7180

42. Which of the following is not required in the preparation of a recombinant DNA molecule?

  • (A) Restriction endonucleases
  • (B) DNA ligase
  • (C) DNA fragments
  • (D) E. coil
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ID- 7181

43. In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

  • (A) charge only
  • (B) size only
  • (C) charge to size ratio
  • (D) all of the above
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ID- 7182

44. Which isolation DNA from bacteria, which of the following enzymes is not used?

  • (A) Lysozyme
  • (B) Ribonuclease
  • (C) Deoxyribonuclease
  • (D) Protease
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ID- 7183

45. Which of the following has popularised the PCR (polymerase chain reactions)?

  • (A) Easy availability DNA template
  • (B) Availability of synthetic premiers
  • (C) Availability of cheap deoxyribonucleotides
  • (D) Availability of 'thermostable' DNA polymerase
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ID- 7184

46. The role of DNA ligase in the construction of recombinant DNA molecule is:

  • (A) formation of phosphodiester bond between two DNA fragments
  • (B) formation of hydrogen bonds between sticky ends of DNA fragments
  • (C) ligation of all purine and pyrimidine bases
  • (D) none of the above
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ID- 8153

47. Which of the following is not a source of restriction endonucleases?

  • (A) Haemophilus influenza
  • (B) Escherichia coil
  • (C) Entamoeba coil
  • (D) Bacillius amyloliquefaciens
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ID- 8154

48. Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

  • (A) Denaturation of template DNA
  • (B) Annealing of primers to template DNA
  • (C) Extension of premier end on the template DNA
  • (D) All of the above
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49. Who among the following was awarded the Nobel Prize for the development of PCR technique?

  • (A) Herbert Boyer
  • (B) Hargovind Khurana
  • (C) Kary Mullis
  • (D) Arthur Kornberg
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EXPLANATION:

  • The polymerase chain reaction or PCR, as it is commonly called, was originally invented by Kary Mullis in 1985.
  • Kary Mullis shared the Nobel Prize with Michael Smith in Chemistry in 1993.

Therefore, option C is correct.

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ID- 8156
BSEB, 2016

50. Which one of the following is possible in micropro-pagation?

  • (A) Asexual reproduction
  • (B) Multiplication of genetically identical copies
  • (C) Sexual reproduction
  • (D) Both 'A' and 'B'
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ID- 8157
BSEB, 2016 (C)

51. Which of the micro organism is used in alcohol industry?

  • (A) Yeast
  • (B) Lacto bacillus
  • (C) Amoeba
  • (D) P.M.V.
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ID- 8158
BSEB, 2017 (A)

52. Which Bacterium is used in formation of crud from milk?

  • (A) Clostridium
  • (B) Lactobacillus
  • (C) 'A' and 'B' both
  • (D) Streptococcus
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ID- 8159
BSEB, 2018 (A)

53. GAATTC is recognition site for which restriction endonuclease?

  • (A) Hind III
  • (B) Eco RI
  • (C) Bam I
  • (D) Hae III
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ID- 8160
BSEB, 2018 (A)

54. The specific sequence of DNA recognized by Restriction Endonucleases are:

  • (A) Palindromic nucleotide sequence
  • (B) VNTR
  • (C) Minisatellite
  • (D) All of these
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ID- 8161
BSEB, 2018 (A)

55. A molecular technique in which multiple copy of the desired gene is synthesized in vitro is called as:

  • (A) ELISA
  • (B) PCR
  • (C) Gel Electrophoresis
  • (D) Flow cytometry
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ID- 8162
BSEB, 2018 (A)

56. Which of the following enzyme is used to break the bacterial cell wall to release DNA and other bio macro molecules?

  • (A) Lysozyme
  • (B) Cellulase
  • (C) Chitinase
  • (D) Collagenase
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