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Biotechnology And Its Application

Board » Bihar Board » Class 12th » English Medium » Science » Biology » L-12 : Biotechnology And Its Application

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ID- 8378
BSEB, 2020 (A)

1. Which of the following is a plasmid?

  • (A) Bam HI
  • (B) Eco RI
  • (C) pBR322
  • (D) Hind III
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ID- 8379
BSEB, 2015

2. Fusogen of protoplast culture is:

  • (A) liquid nitrogen
  • (B) PEG
  • (C) lactic acid
  • (D) all of these
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ID- 8380
BSEB, 2011, 2020

3. Which vitamin is found in golden rice?

  • (A) B12
  • (B) A
  • (C) D
  • (D) C
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ID- 8381

4. Transgenic Golden Rice is enriched with high:

  • (A) Lysine
  • (B) Methionine
  • (C) Glutenin
  • (D) Vitamin A
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ID- 8382

5. Reagent used in ELISA test is:

  • (A) Endonuclease
  • (B) Polymerase
  • (C) Ligase
  • (D) Peroxidase
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ID- 8383

6. In Bt cotton, a transgenic plant, Bt refers to:

  • (A) Notanical
  • (B) Bacillus thuringiensis
  • (C) Beta
  • (D) Biotechnology
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ID- 8384

7. Recombinant DNA technology discovered by:

  • (A) Hargobind Khoorana
  • (B) James D. Watsan
  • (C) S. Kohen and H. Boyer
  • (D) Sulton and Boveri
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ID- 8385

8. Biopiracy is related to the following:

  • (A) Traditional knowledge
  • (B) Biomolecules and Bioresources, Isolation of gene from Bioresources
  • (C) Bioresources
  • (D) All of the above
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ID- 8386
BSEB, 2018 (C)

9. The downstream processing of biosynthetic products before is ready for marketing as a finished product usually includes:

  • (A) Separation
  • (B) Purification
  • (C) Both 'A' & 'B'
  • (D) None of these
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ID- 8387
BSEB, 2018 (C)

10. Which of the following is not considered as vector for DNA cloning?*

  • (A) BAC and YAC
  • (B) Expression vector
  • (C) T DNA
  • (D) None of these
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ID- 8388

11. First Transgenic crop was:

  • (A) Cotton
  • (B) Alsi
  • (C) Pea
  • (D) Tobacco
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ID- 8389

12. Adjoining of exotic gene in crop is called:

  • (A) Genetic Engineering
  • (B) Bio architecture
  • (C) Tissue culture
  • (D) Immunisation
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ID- 8390

13. Which one of the following is a step of PCR?

  • (A) Denaturation
  • (B) Annealing
  • (C) Extension
  • (D) All of these
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ID- 8391
BSEB, 2015, 2020 (A)

14. The Ti plasmid used in genetic engineering is obtained from:

  • (A) Bacillus thruingiensis
  • (B) E. coil
  • (C) Agrobacterium rhizogenes
  • (D) Agrobacterium tumefaciens
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ID- 8392

15. From which of the following plants, the insecticide pyrethrum is prepared:

  • (A) Cymhopogen
  • (B) Tephrosia
  • (C) Chrysanthemum
  • (D) Vetivera
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ID- 8393

16. The sheep 'Dolly' was cloned by using somatic cells from donor's:

  • (A) Udder
  • (B) Skin
  • (C) Tongue
  • (D) Ear lobe
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ID- 8394

17. Natural genetic engineer is:

  • (A) Bacillus subtilis
  • (B) Pseudomonas sp
  • (C) Escherichia coil
  • (D) Agrobacterium tumefaciens
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ID- 8395

18. Anticoagulant hirudin is found in:

  • (A) In Snake
  • (B) In Lizard
  • (C) In Leechi
  • (D) In Scorpion
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ID- 8396

19. Herbicide resistant gene in plant is:

  • (A) Ct
  • (B) Mt
  • (C) Bt
  • (D) Gst
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ID- 8397

20. Most widely used bioweapon is:

  • (A) Bacillus subtilis
  • (B) Psudomonas putida
  • (C) Bacillus anthracis
  • (D) None of the above
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ID- 8398

21. Habitat together with function of species constitute its:

  • (A) Topography
  • (B) Trophic level
  • (C) Boundary
  • (D) Ecological niche
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ID- 8399
BSEB, 2018 (C)

22. If any protein encoding its expressed in heterologous host, then it is called as:

  • (A) Recombinant protein
  • (B) Heterozygous protein
  • (C) Antibiotic
  • (D) None of these
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ID- 8400

23. Which of the following genes where introduced in cotton to protect it from cotton bollworms?

  • (A) Cry Ac and Cry Ab
  • (B) Bt Ac and Bt Ab
  • (C) Cry IAc and Cry II Ab
  • (D) Nif genes
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ID- 8401

24. Bt toxins are:

  • (A) intracellular lipids
  • (B) intracellular crystalline proteins
  • (C) extracellular crystalline proteins
  • (D) interacellular polysaccharides
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ID- 8402
BSEB, 2018 (A), 2021 (A)

25. Which one of the following is not used as bio-fertilizer?

  • (A) Bacillus thuringiensis
  • (B) Anabaena
  • (C) Nostoc
  • (D) Rhizobium
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EXPLANATION:

Bacillus thuringiensis is a gram-positive spore-forming soil microorganism. The toxin produced by Bacillus thuringiensis has been used as an insecticide spray since 1920s and is commonly used in organic farming.

Therefore, option A is correct.

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ID- 8403

26. 'Nif' gene for nitrogen fixation in cereal crops like wheat, jowar etc. is introduced by cloning:

  • (A) Rhizobium meliloti
  • (B) Bacillus thuringiensis
  • (C) Rhizopus stolonifer
  • (D) Agrobacterium tumefaciens
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ID- 8404

27. The transgenic plant 'Flavr Savr' tomato carries an artificial gene for:

  • (A) delayed ripening process
  • (B) longer shelf life
  • (C) enhanced flavour
  • (D) all of these
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ID- 8405

28. Hirudin is:

  • (A) a protein produced by Hordeum vulgare, which is rich in lysine
  • (B) a toxic molecule isolated from Gossypium hirsutum, which reduces human fertility
  • (C) a protein produced from transganic Brassica napus which prevents blood clotting
  • (D) an antibiotic produced by a genetically engineered bacterium Escherichia coli
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ID- 8406

29. A transgenic food crop which may help in solving the problem of night blindless in developing countries is:

  • (A) Bt cotton
  • (B) golden rice
  • (C) flavr savr
  • (D) Bt corn
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ID- 8407
BSEB, 2018 (A)

30. Which of the following is the nematode that attacks the roots of tobacco plants?

  • (A) Agrobacterium tumefaciens
  • (B) Rhizobium leguminosarum
  • (C) Meloidogyne incognita
  • (D) Taenia solium
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ID- 8408

31. First genetically modified plant commercially released in India is:

  • (A) golden rice
  • (B) flavr savr
  • (C) Bt-brinjal
  • (D) Bt-cotton
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ID- 8409

32. Adenosine deaminase deficiency can be permanently cured by which of the following methods?

  • (A) Bone marrow transplantation
  • (B) Enzyme replacement therapy
  • (C) Gene therapy at early embryonic stages
  • (D) All of these
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ID- 8411

33. Technique used to detect the DNA in a clone is:

  • (A) polymerase chain reaction
  • (B) gel electrophoresis
  • (C) chromatography
  • (D) autoradiography
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ID- 8413

34. It is a single standed DNA or RNA, tagged with a radioactive molecule and is used to detect mutated genes:

  • (A) RNAi
  • (B) Probe
  • (C) Plasmid
  • (D) Primer
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ID- 8415

35. Which of the following companies started selling humulin in the year 1983?

  • (A) Eli Lilly
  • (B) Genetech
  • (C) GEAC
  • (D) None of these
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ID- 8416

36. The study of all the proteins coded by the genome is called as:

  • (A) proteomics
  • (B) genomics
  • (C) gene library
  • (D) proteology
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ID- 8420

37. Gene therapy can be referred to as:

  • (A) pre-clinical testing for inherited diseases in newborns
  • (B) treatment of diseases caused by genetic defect
  • (C) genetic engineering using rDNA technology
  • (D) cancer treatment using in vitro cultured stem cells
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ID- 8422

38. Human insulin is being commercially produced from a transgenic species of:

  • (A) Mycobacterium
  • (B) Rhizobium
  • (C) Saccharomyces
  • (D) Escherichia
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ID- 8423

39. During the processing of proinsulin into the mature insulin:

  • (A) C-peptide is added to proinsulin
  • (B) C-peptide is removed from proinsulin
  • (C) B-peptide is added to proinsulin
  • (D) B-peptide is removed from proinsulin
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ID- 8425

40. Second generation vaccines are prepared by recombinant DNA technology. Which out of the following are the examples of such vaccines?

  • (A) Hepatitis B virus vaccine
  • (B) Herpes virus vaccine
  • (C) Salk's polio vaccine
  • (D) Both 'A' and 'B'
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ID- 8428

41. Dolly sheep was genetically similar to:

  • (A) the mother from which enucleated egg cell was taken
  • (B) the mother from which nucleated udder cell was taken
  • (C) the surrogate mother
  • (D) both surrogate mother and donor mother
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ID- 8430

42. The transgenic animals are those which have:

  • (A) foreign RNA in all its cells
  • (B) foreign DNA in some of its cells
  • (C) foreign DNA in all its cells
  • (D) both 'A' and 'C'
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ID- 8434

43. Which Indian plants have either been patented or attempts have been made to patent them by western nations for their commercial use?

  • (A) Basmati rice
  • (B) Turmeric
  • (C) Neem
  • (D) All of these have been targeted
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ID- 8436

44. Biopiracy means:

  • (A) ise of biopatents
  • (B) thefts of plants and animals
  • (C) stealing of bioresources
  • (D) exploitation of bioresources without authentic permission
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ID- 8439

45. Which of the following has been covered under the broad patent category?

  • (A) Triticum
  • (B) Oryza
  • (C) Pisum sativum
  • (D) Brassica
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ID- 8442

46. Bt cotton is not:

  • (A) a GM plant
  • (B) insect resistant
  • (C) a bacterial gene expressing system
  • (D) resistant to all pesticides
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ID- 8448

47. C-peptide of human insulin is:

  • (A) a part of mature insulin molecule
  • (B) responsible for formation of disulphide bridges
  • (C) removed during maturation of pro-insulin
  • (D) responsible for its biological activity
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ID- 8451

48. GEAC stand for:

  • (A) Genome Engineering Action Committee
  • (B) Ground Environment Action Committee
  • (C) Genetic Engineering Approval Committee
  • (D) Genetic and Environment Approval Committee
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ID- 8461

49. α-1 antitrypsin is:

  • (A) an antacid
  • (B) an enzyme
  • (C) used to treat arhritis
  • (D) used to treat emphysema
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ID- 8469

50. A probe which is a molecule used to locate a specific sequences in a mixture of DNA or RNA molecules could be:

  • (A) a single stranded RNA
  • (B) a single standed DNA
  • (C) either RNA or DNA
  • (D) can be ss DNA but not ss RNA
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ID- 10555

51. The site of production of ADA in the body is:

  • (A) erythrocytes
  • (B) lymphocytes
  • (C) blood plasma
  • (D) osteocytes
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ID- 10556

52. A protoxin is:

  • (A) a primitive toxin
  • (B) a denatured toxin
  • (C) toxin produced by protozoa
  • (D) inactive toxin
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ID- 10557

53. Pathophysiology is the:

  • (A) study of physiology of pathogen
  • (B) study of normal physiology of host
  • (C) study of altered physiology of host
  • (D) none of the above
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ID- 10558

54. In RNAi, gene are silenced using:

  • (A) ss DNA
  • (B) ds DNA
  • (C) ds RNA
  • (D) ss RNA
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ID- 10559
BSEB, 2018 (A)

55. The first clinical gene therapy was done for the treatment of:

  • (A) AIDS
  • (B) cancer
  • (C) cystic fibrosis
  • (D) SCID (Sever Combined Immuno Deficiency resulting from deficiency of ADA)
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ID- 10560

56. ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?

  • (A) Adenosine deoxy aminase
  • (B) Adenosine deaminase
  • (C) Aspartate deaminase
  • (D) Arginine deaminase
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ID- 10561
BSEB, 2018 (A)

57. Silencing of a gene could be achieved through the use of:

  • (A) RNAi only
  • (B) antisense RNA only
  • (C) both RNAi and antisense RNA
  • (D) none of the above
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ID- 10562
BSEB, 2016, 2021 (A)

58. 'Cry gene' prevents which crop from bollworms?

  • (A) Cotton
  • (B) Mango
  • (C) Tea
  • (D) Wheat
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ID- 10563
BSEB, 2016

59. Which type of bonding is present in between nitrogenous bases of nucleic acid?

  • (A) Peptide bond
  • (B) Ester bond
  • (C) Both 'A' and 'B'
  • (D) Hydrogen bond
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ID- 10564
BSEB, 2016

60. Artificial seed is formed which the help of:

  • (A) Somatic embryo
  • (B) Polyembryony
  • (C) Both 'A' and 'B'
  • (D) None of these
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ID- 10565
BSEB, 2018 (A)

61. The toxic protein secreted by Bacillus thuringiensis is:

  • (A) Tubulin
  • (B) Insulin
  • (C) Cry protein
  • (D) All of these
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ID- 10566
BSEB, 2019 (A)

62. ampR gene is responsible for developing resistance in:

  • (A) Pest
  • (B) Insect
  • (C) Antibiotic
  • (D) Drought
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ID- 10567
BSEB, 2019 (A)

63. Transgenic mice may be used for testing of:

  • (A) The safety of vaccines
  • (B) Efficiency of fertilizers
  • (C) Doses of antibiotics
  • (D) All of these
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